Osteoblastic cell behavior to tantalum coating on Ti-6Al-4V alloy
이보아, 차준영, Piao Xing-Hui, 이현주, 김영준,
소속 상세정보
이보아 ( Lee Bo-Ah ) - Chonnam National University School of Dentistry Department of Periodontology
차준영 ( Cha Jun-Young ) - Chonnam National University School of Dentistry Department of Periodontology
( Piao Xing-Hui ) - Chonnam National University School of Dentistry Department of Periodontology
이현주 ( Lee Hyun-Joo ) - Chonnam National University School of Dentistry Department of Periodontology
김영준 ( Kim Young-Joon ) - Chonnam National University School of Dentistry Department of Periodontology
KMID : 0353420160400030117
Abstract
Tantalum (Ta) coating is expected to improve clinical outcomes of titanium (Ti) dental implants due to its anticorrosion and biocompatibility. The purpose of this study was to evaluate the effect of Ta coating on surface characteristics and osteoblastic cell response of Ti-6Al-4V alloy surface. Radio frequency (RF) magnetron sputtering method was used to develop the Ta coating layer on Ti- 6Al-4V disks. Surface properties were analyzed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Electrochemical test was performed to evaluate the corrosion behavior. Cell responses were evaluated using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay and alkaline phosphatase (ALP) activity. Ta coating layer was formed with a uniform thickness. In electrochemical test, the passive layer was more uniform and stable in Ta-coating group than Ti-6Al-4V group. Cell morphology showed no difference between Ti-6Al-4V group and Ta-coating group. Ta-coating group showed higher cell proliferation than Ti-6Al-4V group at 1, 3 and 5 days (p<0.05). There was no significant difference in ALP activity level between two groups (p>0.05). These results suggest that Ta coating on Ti-6Al-4V alloy could enhance surface properties and early osteogenic responses.
키워드
Biocompatible materials; Cell proliferation; Surface properties; Tantalum; Titanium
원문 및 링크아웃 정보
등재저널 정보